Estimation of Cefoperazone in marketed formulation by RP-HPLC

A RP-HPLC assay method has been developed and validated for cefoperazone. An isocratic RP-HPLC was developed on a SS Wakosil IIC18 column (250 mm × 4.6 mm i.d., 5 μm) using a mobile phase of phosphate buffer (pH 6.8) and methanol (3:1 v/v) with UV detection at wavelength 254 nm at the flow rate 1 ml/min. The proposed method was validated for sensitivity, selectivity, linearity, accuracy, precision, ruggedness, robustness and solution stability. The response of the drug was linear in the concentration range of 0.4 -100 μg/ml. Limit of detection and Limit of quantification was found to be 0.2 μg/ml and 0.4 μg/ml respectively. The % recovery ranged within 95-106 %. Method, system, interday and intraday precision was found to be within the limits of acceptance criteria. Method was found to be rugged when different analyst carried out analysis. The method was found to be sensitive and efficient with 3370.78 theoretical plates, 0.2966 mm HETP and tailing factor 1. The method was suitable for the quality control of cefoperazone in injection formulations.